| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Cloning and expression analysis of allograft inflammatory factor type 1 in coelomocytes of antarctic sea urchin (Sterechinus neumayeri)
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| Autores: |
Ovando, Fernanda
Gimpel, Carla
Cardenas, Constanza
Machado Cunha Da Silva, Jose Roberto
De Lorgeril, Julien
Gonzalez, Marcelo
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| Data: |
2012-08
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| Ano: |
2012
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| Palavras-chave: |
Antarctica
Sea urchin
Sterechinus neumayeri
Coelomocytes
Gene expression
AIF-1
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| Resumo: |
We have cloned and characterized for the first time an allograft inflammatory factor 1 (Sn-AIF-1) from the Antarctic sea urchin. We report the cloning of Sn-AIF-1 cDNA and the characterization of its expression in coelomocytes after a bacterial challenge. The cDNA Sn-AIF-1 has a size of 608 bp and encodes a polypeptide of 151 aa. The deduced amino acid sequence has a putative size of 17.430 Da, an isoelectric point of 4.92, and shows 2 elongation factor handlike motifs that normally bind calcium ions. BLAST analysis revealed close matches with other known AIF-1. The deduced amino acid sequence of Sn-AIF-1 showed high homology with AIF-1 in vertebrates such as fish, mice, and humans; and in the case of invertebrates, the major degree of identity (55%) was with a predicted sequence of the purple sea urchin AIF-1, and 52% corresponded to a sponge. Expression of Sn-AIF-1 mRNA was analyzed by qPCR. Sn-AIF-1 mRNA expression was measured from coelomocytes after a bacterial challenge using RT-PCR and revealed that the gene was upregulated after 24 h. Sn-AIF-1 could participate in the inflammatory response, particularly in the activation of coelomocytes and their survival.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00098/20955/18572.pdf
DOI:10.2983/035.031.0336
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| Editor: |
Natl Shellfisheries Assoc
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| Relação: |
http://archimer.ifremer.fr/doc/00098/20955/
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| Formato: |
application/pdf
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| Fonte: |
Journal Of Shellfish Research (0730-8000) (Natl Shellfisheries Assoc), 2012-08 , Vol. 31 , N. 3 , P. 875-883
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| Direitos: |
2012 BioOne All rights reserved, National Shellfisheries Association
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